Biology Projects for New Members
Below is a list of Biology Training Project to become familiar with sample preparation, All the training will be done at Knudsen A-154, and you must take the Lab Safety Fundamental Course, before you enter into A-154.
[B1] Project #B1 - Pick up C.elegans (Week 1-2)
[B2] Project #B2 - Make agarose gel (Week 1-2)
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[B1] Project #B1 - Pick up C.elegans (Week 1-2)
- Now, it's time to learn how to handle C. elegans
- First, please watch their behavior under a microscope as long as you wish.
- Gently pick up C. elegans and place it back.
- Pick C. elegans and move to a different petri disk.
- Practice many times as many as you like. (This process may take several hours. Be patient!)
- Please observe carefully how they behave. Identify several different type of motions (such as forward, backward, pause, turn left, turn right. ) What kind of external stimulations make them react? Please keep writing all of your findings. Your careful observation here is going to become very useful to become a good scientist!
- Write one-page report and post it at CCLE.
[B2] Project #B2 - Make agarose gel (Week 1-2)
- Agarose gel is one of the basic components of samples. To practice, we will be making 2% agar solution.
- First, take a look at this link to get the procedure. Feel free to refer to it whenever you want.
- Now practice making some agar. As you do, observe the color and consistency of your product. Is it smooth? Chunky? Bubbly? Make note of what it looks like.
- your product should be smooth, slightly opaque, and without bubbles. It should not have any loose flecks of powder.
- Label your product with the date and concentration and put it in the fridge.
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