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Interesting Movies, Video Lectures


A Brief Introduction to C. Elegans (by OpenWorm)


iBioSeminars

iBioSeminars.org has been developed to bring the best biology to people throughout the world for free, medical, chemical and physical research in a video format.
Here are some examples:

[1] Neuroscicence:
  • Cori Bargmann Part 1: Genes, the brain and behavior
  • Cori Bargmann Part 2: Cracking the circuits for olfaction: odors, neurons, genes and behavior
  • Eve Marder : Introduction to Central Pattern Generators
  • Jeff Lichtman :  Connectomics
[2] Genetics:
  • Melissa Moore : Split Genes and RNA Splicing
  • Susan Wessler : Introduction to transposable element
  • Jonathan Weissman : DNA Sequencing
[3] Microscope and Technique:
  • Kurt Thorn: Confocal Microscopy
  • Nico Stuurman: Fluorescence Microscopy

Journal of Visualized Experiments (JoVE)

JoVE contains many useful movies. Here is the list of movies related to C. elegans.

[1] Behavior Experiments:
  • C. elegans Tracking and Behavioral Measurements
  • Microfluidic-based Electrotaxis of C. elegans
  • C. elegans Chemotaxis Assay
  • C. elegans Positive Butanone Learning,Short-term, and Long-term Associative Memory Assays
  • A Molecular Readout of Long-term Olfactory Adaptation in C. elegans
[2] Experimental Techniques:
  • In vivo Neuronal Calcium Imaging in C. elegans
  • In vivo Laser Axotomy in C. elegans
  • Dissecting and Recording from The C. Elegans Neuromuscular Junction
[3] Embryonic Development:
  • C. elegans Life Span in 96 Well Microtiter Plates
  • Imaging C. elegans Embryos using an Epifluorescent Microscope
  • Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
  • Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos
  • Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
[4] Genetic Study:
  • Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
  • Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
[5] Amylopathy Study:
  • C. elegans Model System for Amylopathy Study

Novel Lectures

Nobel lectures by the researches of C. elegans can be found in Worm History in WorbBook.
  • Nature's gift to science (2002) - Sydney Brenner 
  • Worms, life and death (2002) - H. Robert Horvitz 
  • C. elegans: the cell lineage and beyond (2002) - John E. Sulston
  • Gene silencing by double stranded RNA (2006) - Andrew Z. Fire 
  • Return to the RNAi world (2006) - Craig C. Mello 
  • GFP: Lighting up life (2008) - Martin Chalfie 

Embryonic development of C. elegans

Development of a C. elegans embryo from the first cell division until hatching.
The movie was acquired at a temperature of 20C using DIC optics. 

Asymmetric Cell Division in Early Embryo

C. elegans embryo carrying GFP-PH and Histone- mCherry imaged from single-cell stage post-fertilization. The migration of female pronucleus towards the male pronucleus is followed by nuclear envelope breakdown, segregation of chromosomes and asymmetric cell division. These early asymmetric division defines the three principal body plane axes namely anterior-posterior, dorso-ventral, and left-right. The first asymmetric division defines the anterior- posterior axis with Anterior to the left of the frame and posterior to the right. At the four cell stage, the bottom of the frame is destined to be the dorsal side while the top part becomes the ventral side of the organism. (Courtesy of Ronen lab.)

Mating Behavior

The movie below shows mating behavior of C. elegans. This is their most complex behavior, programmed in a handful neurons in a male C. Elegans.

P. pacificus eats C. elegans

Pristionchus pacificus is a nematode that can take advantage of many different food sources. In this video, it can be seen utilizing the model organism Caenorhabditis elegans as prey. A small tooth in the mouth opening punctures the cuticle of its prey, then it sucks in the contents. This predatory feeding behavior represents a more complex palette of behavior than C. elegans, but the nervous systems are composed of the same neurons. I am inter

Harlem Shake of C.  elegans

Last Edited by Katsushi Arisaka on March 2, 2022